Identification of Microbes Isolated from Pig Organs Using Morphologic-Biochemical Methods and API Test

Abstract

The study aims to isolate microbes residing within the internal organs of healthy pigs, extracted from the skin.
Through rigorous examination, we aim to analyze their morphological, tinctural, and biochemical characteristics, utilizing
the API test to classify the types and subtypes accurately. Additionally, we seek to isolate museum strains for further
investigation. Moreover, we intend to document the prevalence and frequency of detection of various microbial types within
the sampled specimens. In the course of microbiological examination, 76 samples were obtained from the internal organs
of healthy pigs. The analysis revealed the isolation of 8 subtypes of staphylococci and 7 types of enterobacteria, each with
their respective subtypes. Utilizing morphological, tinctural, biochemical, and API test data for the isolated microbes, we
achieved a confidence level of 99.9 % in accurately determining the type and subspecies of each microbe. Based on the
comprehensive data, we selectively preserved strains, including 5 subtypes of staphylococci and 5 types of enterobacteria
with their subtypes.