The Creation of a Gene Library of Valuable Plant Species in Armenia

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  • M.V. Badalyan, T.B. Aloyan, A.H. Barseghyan, A.Sh. Melikyan

Բանալի բառեր-:

genomic DNA, gene library, genetic transformation, ligase enzyme, restriction enzyme

Ամփոփում

Using DNA banks or gene libraries is the most efficient method of protecting
and managing valuable, endangered, and rare gene pools. Genomic libraries are
collections of bacteria colonies with restriction sites on their genomic DNA that
contain desired genes from a specific organism. The “Scientific Center of Agro-
Biotechnology” branch of ANAU has created gene libraries of 34 plant species,
which are preserved in the National Genebank of Crops and their Wild Relatives,
which forms a basis for genomic selection. This allows genetic identification,
barcoding, DNA formula decoding, and, most importantly, is the basis for genome
identification. Based on the results, plasmids can incorporate restriction segments
of genomic DNA and be efficiently transformed. The high level of correlation
between the number of necessary clones and the efficiency of transformation of
all studied plant species proves the completeness of the gene pool.

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2023-09-26