Dietary Trials and Gut Candidate Phyla Radiation Bacteria: The Effect of Placebo on the Prevalence of Saccharibacteria in Healthy Armenian Women and Women with Familial Mediterranean Fever
DOI:
https://doi.org/10.52276/25792822-2024.4-345Keywords:
Candidate Phyla Radiation, placebo, Saccharibacteria, FMF, microbiotaAbstract
“Candidate Phyla Radiation” (CPR) bacteria, representing ~15 % of bacterial diversity and over 70 phyla, are extremely small bacteria that primarily survive in parasitic or symbiotic forms. CPR bacteria, including Candidatus Brownbacteria, Candidatus Hugbacteria, and Candidatus Saccharibacteria (formerly TM7), were first identified in humans in 2007. They are linked to the microbiota of healthy and diseased individuals, being present in the oral cavity, gastrointestinal, and reproductive tracts. CPR bacteria, such as Saccharibacteria, are associated with dysbiotic conditions like periodontitis and can act as pathogens and potential protectors against inflammatory damage caused by host-associated bacteria.This study aimed to assess the effect of a placebo on gut Saccharibacteria in healthy Armenian women and those with Familial Mediterranean Fever (FMF) disease, a condition with high prevalence in Armenia and often associated with oral microbiota disturbances. Stool samples were analyzed using a culture-independent, high-density DNA microarray method, and statistical analyses were performed with Multibase 2015 Excel Add-in program (NumericalDynamics, Tokyo, Japan). Results indicate that Saccharibacteria respond variably to placebo depending on health status, with some showing significant quantitative or qualitative changes while others remained unchanged. In conclusion, this study confirms the presence of CPR bacteria in the gut microbiota of both healthy women and those with FMF. The distinct responses of intestinal CPR bacteria to placebo highlight the importance of placebo-controlled trials in microbiota research. Furthermore, the findings emphasize the potential role of Saccharibacteria in gut-brain processes and their implications in health and disease.
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Copyright (c) 2025 Natalya Harutyunyan, Lena Stepanyan, Lena Malkhasyan, Astghik Pepoyan

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